Himachal Journal of Agricultural Research

Mandeep Sharma ¹, Aneesh Thakur ¹, Prasenjit Dhar ¹, Rajesh Chahota ¹, Shailja Katoch ¹, Subhash Verma ¹

Affiliations
1 Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN

Abstract

Pasteurella multocida is responsible for major animal diseases of economic importance in both developed and developing countries including India. The prevalence of pasteurellosis has been documented in India as well as in the state of Himachal Pradesh where disease outbreaks from different agro-climatic regions have been reported. Aiming at increased knowledge of these infections in Himachal Pradesh, we employed the conventional methods and advanced molecular methods for culturing, characterization, capsular serotyping, and analysis of virulence factors for rapid epidemiological investigation and pathogenicity of Pasteurella multocida infections in H.P. Vaccine-induced protective biomarkers were also studied using advanced immunological techniques. We also summarize our efforts to enhance the ability to rapidly diagnose clinical infections and to prevent and control pasteurellosis by validation of vaccines.

Keywords

Haemorrhagic Septicaemia, Pasteurella multocida, Diagnostics, Typing, Virulence Factors, Vaccines.

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Jyoti Jaryal ¹, Ajay Katoch ¹, Sanjeev Kumar ¹, Kritika Dhial ¹, Mandeep Sharma ¹, Prasenjit Dhar ¹, Rajesh Chahota ¹, Subhash Verma ¹

Affiliations
1 Department of Veterinary Microbiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, IN

Abstract

In this study, a total of 57 blood samples from cows and buffaloes showing clinical signs of high fever, anemia and/or swollen lymph nodeswere screened for haemoprotozoan infections using polymerase chain reaction (PCR). Some asymptomatic animals were also included in the study. Out of 57 blood samples, 14 samples were positive for either one or the other haemoprotozoa reflecting an overall prevalence of 24.56%. A total of nine samples of which six from cows and three from buffaloes were positive for Theileria annulata and five samples all from cows were found positive for mixed infection of Babesia bovis and Anaplasma marginale. This study shows that haemoprotozoan infections are common in cows and buffaloes population of Kangra District of Himachal Pradeshdue to conduciveenvironmental conditions for survival of vectors responsible for carrying these infectious agents. PCR technique showed high sensitivity and specificityup to species level, as it not only detected the haemoprotozoan infections in active cases but also in asymptomatic carrier animals.

Keywords

Anaplasma marginale, Babesia bovis, Bovine Haemoprotozoan, Himachal Pradesh, Kangra, PCR.

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Tania Gupta ¹, Vanita Patial ¹, Shivani Angaria ¹, Diksha Bali ¹, Mandeep Sharma ¹, Rajesh Chahota ¹

Affiliations
1 Department of Veterinary Microbiology, Dr. G.C.Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN

Abstract

Capripoxvirus, belong to Poxviridiae family, cause highly contagious disease of sheep, goat and cattle. Sheep and goat husbandry, mostly migratory type, is practised by many farmers, which contribute significantly to their income. Sheep and goat pox infections are economically important diseases leading to short- and longterm losses to the farmers. A steep rise in sheep and goat pox outbreaks has been recorded in Himachal Pradesh in last few years, therefore, this review summarises recent advances in the epidemiology, pathology and diagnostics of sheeppox and goatpox diseases. This will be helpful in implementing improved disease management strategies in Himachal Pradesh.

Keywords

Capripoxvirus, Economic Burden, Goat, Sheep.

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Tania Gupta ¹, Vanita Patial ¹, Prasenjit Dhar ¹, Subhash Verma ¹, Mandeep Sharma ¹, Rajesh Chahota ¹

Affiliations
1 Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur-176 062, IN

Abstract

Sheeppox is a highly contagious disease of sheep causing huge economic losses to the sheep industry and farmers rearing the livestock. Himachal Pradesh has large population of sheep reared by the different rural communities. In recent years, outbreaks of sheep pox have become frequent causing huge economical losses to sheep breeders. Isolation, identification and characterization of circulating Sheeppox virus (SPV) strains is required to initiate efforts for disease control and prevention. The aim of the present study was to establish the best cell culture-based isolation system by comparing the growth of SPV field strains from Himachal Pradesh in lamb testicular (LT) cells and continuous Vero cell line. The results showed that LT cell culture is more suited for primary isolation of SPV field strains as compared to Vero cells. But virus can be adapted to Vero cell line after 5 blind passages. Thus, it was concluded that LT cell culture, though tedious, should be preferred for primary isolation of SPV field strains.

Keywords

Sheeppox Virus, LT Cell Culture, Vero Cell Line.

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